We used the variant hemoglobin testing system variant ii beta thalassemia short program, bio rad laboratories inc. Biorad hemoglobins portal biorad library of variants. Hemoglobin a1c, the bestdefined of these, is formed by the reversible condensation of the carbonyl group of glucose and the amino group at the nterminus of the beta chain of hemoglobin. Learn more about the benefits of using bio rad a1c and the impact of hb variants on hba1c results. Performances of capillary electrophoresis and hplc methods in. Mar 28, 2014 the aim of this study is to compare a capillary electrophoresis method and two high. The variant ii turbo hemoglobin testing system is a fully automated, highthroughput hemoglobin analyzer. We wished to introduce the variant ii to eliminate the manual predilution step required by the variant. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Dna analysis kits and agarose gel electrophoresis kits. The variant ii hba1c program has been certified by the national. Variant ii turbo hemoglobin testing system clinical.
Those samples were also analyzed by capillary electrophoresis capillarys 2. Variant iiturbo hemoglobintestingsystem biorad laboratories hemoglobin testing variant ii turbo hemoglobin testing system delivers high throughput and high quality in a single platform powerful software clinical data management cdm software manages and evaluates sample data qc module tracks quality control. No result was given for hba 1c determination in both patients with hb. Precision studies were performed using the biorad lyphochek diabetes control biorad laboratories. Welcome to the biorad library of variants a compendium of hplc cases. Reagents and supplies for hemoglobin a1c testing on the variant ii turbo. In this study, we compared the bio rad hba 1c dual kit assay, recently introduced in canada, on the variant ii analyzer variant ii with the bio rad variant i semiautomated hplc method variant i currently available at tml. The variant ii hemoglobin a 1c program reorder pack is a fully automated assay using hplc technology to deliver precise and accurate hba 1c results. Powered by intelligent automation, this multidisciplinary automation solution. The variant ii turbo hemoglobin a1c program utilizes principles of ion. The report prepared incorporates numerical as well as graphical data, and their analysis is discussed next. We are offering trusted quality products hemocue wbc diff system, alere rapid dengue test kit, alere sd bioline hcv test kit, hbsag alere abbott determine, ichoroma ii advanced portable immune analyzer. The variant ii hemoglobin testing system is flexible, efficient and uses.
At biorad were committed to helping you make a difference in your patients lives. The biorad d10 hemoglobin a1c program is intended for the percent determination of hemoglobin a1c in human whole blood using ionexchange highperformance liquid chromatography hplc. Evaluation of four hemoglobin separation analyzers for. Hemoglobin a1c test system pdj, lcp class ii 21 cfr 862. Detection of abnormal hemoglobin variants by hplc method. Results are processed by biorads clinical data management software cdm. It combines gold standard technology with innovative design and. The eluted hemoglobin fractions are detected by a flowcell type. Biorad d10 hemoglobin testing system lab briefings webinar series cardinal health lab briefings is an educational webinar series that provides clinical insights, industry information, and hot topic. It combines gold standard technology with innovative design and ease of use to enhance medium to high volume laboratory workflow, delivering precise and accurate results. Hplc retention time as a diagnostic tool for hemoglobin variants. Glycosylated hemoglobin hba 1c was determined using the biorad variant ii analyzer biorad laboratories, inc. In addition, a personal computer is used to control the variant ii system using clinical data management cdm. Silent haemoglobin variants and determination of hba1c.
Used biorad variant ii vss vcs hemoglobin testing system. The bio rad variant ii turbo chemistry analyzer is a fully automated variant ii hemoglobin testing system that is designed for high volume, featuring positive specimen identification, lis interface, and complete, readytouse test kits for precise and accurate results. The aim of this project was to evaluate the performance of a new hba1c poct analyser, the bio rad in2it, and compare it with the siemens dca 2000, bio rad variant ii and roche tinaquant hba1c gen 2 assay on the cobas c501. The sebia capillarys 2 analyzer, software version 6.
The biorad variant ii turbo chemistry analyzer is a fully automated variant ii hemoglobin testing system that is designed for high volume, featuring positive specimen identification, lis interface, and. Please inquire with any questions about this instrument or pricing. Variant technology provides a comprehensive solution for hba 1c testing. The variant ii turbo hemoglobin testing system combines biorads hplc precision and. Evaluation of the bio rad variant beta thalassemia short program. Evaluation of the in2it analyzer for hba 1c determination. Here is a biorad variant ii vss module powering on and testing itself. The variant ii turbo hemoglobin testing system combines biorads hplc precision and variant detection with fast throughput to provide a comprehensive solution for hba 1c testing.
Variant ii hemoglobin testing system from biorad, digital. Safety precautions follow all procedures and policies in the fairviewuniversity medical center laboratory safety manual. Quantification of hba2 in patients with and without. Biorad hplc because every patient deserves accurate, reliable results, biorad has developed solutions for any workload and testing environment. The variant ii hemoglobin testing system and accessories offer fully automated high volume hemoglobin a 1c and. Biorad d10 hemoglobin testing system cardinal health. Variant ii hemoglobin testing system biorad laboratories. Hba 1c results obtained with the in2it analyzer were compared with the results obtained with ionexchange highperformance liquid chromatography hplc, used routinely in the laboratory for hba 1c assay variant ii, kit 270. The variant ii beta thalassemia short program utilizes principles of ionexchange highperformance liquid chromatography hplc. Designed to the highest standards, the variant ii system is ngspcertified, ce marked to meet the european ivd directive and anchored to the diabetes control and complication trial dcct reference study traceable to the ifcc reference method. Bio rad a1c detects variants that can interfere with immunoassay results. In the two patients with type 2 diabetes and hb graz the chromatogram from the biorad variant ii using the high resolution.
Literature library certificate of analysis qc inserts quality certification. Variant ii betathalassemia short program model numbers 2702103 250 tests and 2702154 500 tests, biorad laboratories, inc. This digital library contains case reports of variants hemoglobin provided by a select group of. The variant ii is a fully automated analyzer that requires no sample preparation and very little. D100 system d100 system and accessories provide the fastest hplc system for hba 1c testing with hemoglobin variants detection. Assay, glycosylated hemoglobin fda 510k applications. Hemoglobin a 1c,hba 2, and hb f quantitation were each eval.
Deming regression analysis gave the following equation variant ii 1. The variant ii turbo hemoglobin testing system combines biorad s hplc precision and variant detection with fast throughput to provide a comprehensive solution for hba 1c testing. Analytical evaluation of hemoglobin a1c dual kit assay on bio. The biorad variant ii hba 1c analyzer biorad, montreal, quebec, canada was used as directed by the manufacturer following a one week training period. Variant ii hemoglobin testing system for hba1c biorad. Offering variant ii hemoglobin testing system from biorad, digital. Hemoglobin fraction analysis by cation exchange highperformance liquid.
Waters hm, howarth je, hyde k, goldstone s, kadkhodaeielyaderani m, cinkotai ki, et al. Used biorad variant ii turbo chemistry analyzer for sale. Pdf evaluation of analytical performance of variant ii. We analyzed all samples on the biorad variant ii hplc system. Confidently report hba 1c results to aid physicians with diagnosing those who have diabetes, identifying those who may be at risk of developing the disease, or monitoring the progress of those living the diabetes. Designed to the highest standards, the variant ii system is ngspcertified, ce marked to. From the hamilton regional laboratory medicine program, hamilton, ontario mr. The kit offers many enhanced attributes such as the ability to report hba 1c in the presence of hbs, hbc, hbd, and hbe. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the. We designed the variant ii turbo link hemoglobin testing system.
Biorad works closely with the worldwide community of laboratories actively working. Visual examination of the elution pattern for each specimen is required and is particularly important for laboratories with a patient population at high risk for. We use the very widely applied biorad variant ii turbo output. The variant ii turbo hemoglobin testing system is a fully automated ionexchange hplc system for midto highvolume laboratories. Evaluation of analytical performance of variant ii turbo.
Manufacturer specifications variant ii hemoglobin testing system, bio rad laboratories please note. Regulatory description product code classification regulation section panel. Biorad variant ii turbo chemistry analyzer highlights. Development of a quality assurance tool to detect hb wayne when measuring hba1c on the biorad variant ii turbo 2. We wished to introduce the variant ii to eliminate the manual predilution step required by the variant i. Welcome to the bio rad library of variants a compendium of hplc cases. The biorad d10 hemoglobin a1c program is intended for professional use only. Used biorad variant ii turbo blood analyzer for sale. Rad laboratories propose the variant ii hplc analyzer for hemoglobinopathy screening, but this analyzer was not tested in the current study. We used the variant hemoglobin testing system variant ii beta thalassemia short program, biorad laboratories inc. Biorad laboratories manufacturer specifications for variant ii hemoglobin testing system glycohemoglobin analyzer on medwow medical equipment.
The d100 system is the future of a1c testing and offers smart hplc results, variant detection, and can aid in diabetes diagnosis without comprising speed and efficiency. Wholesale trader of bio rad products 12 s ii id centrifuge, eia testing evolis system, pr 4100 absorbance microplate reader and bio rad variant ii turbo chemistry analyzer offered by mittal laboratories, delhi. The lyphophilized material was reconstituted as directed by the manufacturer. The analyzer and the reagents used for this evaluation have been pro vided by biorad laboratories hercules, ca, usa and have been used according to manufacturers instructions. Established in the year 1984, we mittal laboratories are a fast emerging and growing company. The variant ii system and accessories offer fully automated high volume hemoglobin a 1c and. Evaluation of a dual hemoglobin a2a1c quantitation kit on the biorad variant ii automated hemoglobin analyzer. The variant ii hemoglobin testing system offers complete automation, with. The variant ii hemoglobin testing system uses the principals of high performance liquid chromatography hplc for the separation and determination of normal and abnormal hemoglobin. There is still much to learn about the impact of hb variants.
To determine whether confirmation of hb c and hb oarab variants by an alternative method is required when using the bio rad variant ii hplc system. Variant ii instrumentation clinical diagnostics biorad. This digital library contains case reports of variants hemoglobin provided by a select group of reference centers belonging to the worldwide community of laboratories actively working on detection of sickle cell, thalassemia and other hemoglobinopathies. Stephane jaisson, nathalie leroy, emmanuelle guillard. Biorad a1c detects variants that can interfere with immunoassay results. The effect of hbs presence in the samples was quantified using the helena sasmx alkaline gel. The variant ii turbo hemoglobin testing system combines biorad s hplc precision and variant detection with fast throughput. Height,21 inches,width,40 inches,year of manufacture,2009,depth,24. The instrument, known as bio rad variant ii beta thalassemia short program utilizes the principle of hplc. Evaluation of a dual hemoglobin a2a1c quantitation.
The kit offers many enhanced attributes such as the ability to report hba 1c in the presence of hbs, hbc, hbd, and hbe traits. Effects of the hb e, hb h and hb gtaichung variants on. False measurement of glycated hemoglobin in patients without. Analytical evaluation of the biorad variant ii automated. Choose from a full menu of accessories for the variant ii hemoglobin. Hplc retention time as a diagnostic tool for hemoglobin. Variant ii hemoglobin testing system from biorad get latest price the variant ii is a fully automated analyzer that requires no sample preparation and very little hands on time by the operator. Biorad provides complete assay kits for analysis of hba 1c and. All analyses were controlled with commercial hb a 1c, hb a 2, and hb f controls purchased from biorad laboratories. Study of hemoglobinopathies and hb variants in population of. Bio rad products 12 s ii id centrifuge wholesale trader. Reagents and supplies for hemoglobin a1c testing on the variant ii turbo system.
Methods sigma levels were calculated using the data obtained from two levels of internal and 12 external quality control materials biorad of variant ii turbo hba1c analyzer. The analyzer integrates and reduces the raw data, and then calculates the relative percentages of each hemoglobin fraction. Nonseparation methods may provide inaccurate results when a patient has a hemoglobin variant. An hb a2f calibrator and two levels of controls bio rad were analyzed at the beginning of each run. Biorad clinical diagnostic solutions hemoglobin a1c us. Hba1c values of 2105 samples, including 37 samples with hb e, h and gtaichung variants identified by hb electrophoresis and the pcr sequence, were evaluated by the ionexchange biorad variant ii turbo analyzer and boronate affinity primus clc 385 analyzer. None of the equipment posted for sale on is owned by medwow, should you have any. The variant ii turbo system and accessories provide fast, fully automated highvolume hemoglobin a 1c testing with clinical data management software for lis. The tests were performed on an instrument manufactured by bio rad laboratories, usa. The aim of this study is to compare a capillary electrophoresis method and two high. The variant ii is a fullyautomated analyzer that requires no sample. Our fullline of automated hemoglobin a1c hba1c testing systems leverage the highly specific and reproducible hplc method to simplify the process of acquiring fast and accurate hba1c results without common hemoglobin variant.
Effect of hbs in the determination of hba2 with the biorad. Manufacturer specifications variant ii turbo hemoglobin testing system, bio rad laboratories please note. Analytical evaluation of hemoglobin a1c dual kit assay on. Dead zone grey area variant ii hemoglobin testing system hemoglobin testing biorad laboratories hemoglobin.
The software controls for overlappingmerging peaks by dropping. The d10 system provides accurate hba1c results in the presence of the most common heterozygous hemoglobin variants. All analyses were controlled with commercial hb a 1c,hba 2, and hb f controls purchased from biorad laboratories. All methods were performed as described in the respective instruction manuals for each kit. Hba1c determined using d10 biorad, usa, variant ii turbo turbo. The secondgeneration instrument, the biorad variant ii, performs the same. Evaluation of a dual hemoglobin a2a1c quantitation kit on. Biorad library of variants biorad hemoglobins portal.
Bricare is available for all of biorads diagnostic instruments for continuous monitoring and remote sessions with an instruments computer. The aim of our study was to evaluate the analytical performance of biorads variant turbo ii hba1c analyzer according to sigma metrics. Todays most demanding laboratories can turn to biorad. Manufacturer specifications variant ii hemoglobin testing system, biorad laboratories please note. The fully automated variant ii hemoglobin testing system is designed for high. Biorad laboratories variant ii hemoglobin testing system. Pointofcaretesting poct of haemoglobin alc hba1c is popular due to its fast turnaround of results in the outpatient setting.
Jan 30, 2019 a total of 40 whole blood samples with edta collected from previously tested specimens were conducted with hba 1c analysis by a biorad variant ii turbo 2. Oct 01, 2004 the clinical data management software does not integrate peaks with elution time shorter than 0. The effect of the presence of hbs in the determination of hba2 using the biorad variant ii analyzer. Precision studies were performed using the biorad lyphochek diabetes control biorad laboratories, montreal, quebec. Variant ii turbo link hemoglobin testing system biorad. Built for exceptional performance, the stateoftheart, fully automated variant ii hemoglobin testing system delivers quality results at. The results are processed by biorads clinical data management cdmtm software.
And variant ii system enables you to be the difference for the patients who count on you most delivering the best chemistry on the market for exceptionally accurate results. Biorad laboratories variant ii turbo hemoglobin testing. D100tm system the d100 tm system consists of a fullyintegrated standalone workstation of automated hplc instrumentation that includes an onboard computer. It consists of two modules the variant ii chromatographic station vcs and the variant ii sampling station vss. Variant ii hemoglobin testing system hemoglobin testing biorad laboratories hemoglobin testing. Hemoglobin testing system variant ii hemoglobin testing. Sebia, lisses, france to confirm the absence of hb variants.
The library of variants is a digital library of hemoglobin variant case reports used for educational purposes only. The variant ii turbo system and accessories provide fast, fully automated highvolume hemoglobin a 1c testing with clinical data management software for lis integration. High volume laboratory hemoglobin a1c analyzer for diabetes includes computer. Biorad laboratories hemlbin testin variant ii hemoglobin testing system quality patient care is at the heart of what you do. Cationexchange highperformance liquid chromatography for. Find new or used biorad variant ii for sale on bimedis. In this study, we compared the biorad hba 1c dual kit assay, recently introduced in canada, on the variant ii analyzer variant ii with the biorad variant i semiautomated hplc method variant i currently available at tml. Evaluation of a dual hemoglobin a2a1c quantitation kit on the bio.
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